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Elabscience Biotechnology lactic acid la colorimetric assay kit
Effect of CB on Glycolysis in M2 Macrophages. ( A ) Fluorescence images of macrophages after uptake of 2-NBDG (400×); ( B ) Quantification of average fluorescence intensity; ( C ) <t>Colorimetric</t> measurement of lactate concentration. ( D – G ) Real-time quantitative PCR analysis of mRNA levels for GLUT1, PKM2, LDHA, and MCT1; ( H – L ) Western blot analysis of protein expression levels for GLUT1, PKM2, LDHA, and MCT1. n=3; * P <0.05, ** P <0.01 compared to M0; # P <0.05, ## P <0.01 compared to M2.
Lactic Acid La Colorimetric Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Guangzhou JET Bio-Filtration l-lactic acid (la) colorimetric assay kit
Effect of CB on Glycolysis in M2 Macrophages. ( A ) Fluorescence images of macrophages after uptake of 2-NBDG (400×); ( B ) Quantification of average fluorescence intensity; ( C ) <t>Colorimetric</t> measurement of lactate concentration. ( D – G ) Real-time quantitative PCR analysis of mRNA levels for GLUT1, PKM2, LDHA, and MCT1; ( H – L ) Western blot analysis of protein expression levels for GLUT1, PKM2, LDHA, and MCT1. n=3; * P <0.05, ** P <0.01 compared to M0; # P <0.05, ## P <0.01 compared to M2.
L Lactic Acid (La) Colorimetric Assay Kit, supplied by Guangzhou JET Bio-Filtration, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of CB on Glycolysis in M2 Macrophages. ( A ) Fluorescence images of macrophages after uptake of 2-NBDG (400×); ( B ) Quantification of average fluorescence intensity; ( C ) <t>Colorimetric</t> measurement of lactate concentration. ( D – G ) Real-time quantitative PCR analysis of mRNA levels for GLUT1, PKM2, LDHA, and MCT1; ( H – L ) Western blot analysis of protein expression levels for GLUT1, PKM2, LDHA, and MCT1. n=3; * P <0.05, ** P <0.01 compared to M0; # P <0.05, ## P <0.01 compared to M2.
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Effect of CB on Glycolysis in M2 Macrophages. ( A ) Fluorescence images of macrophages after uptake of 2-NBDG (400×); ( B ) Quantification of average fluorescence intensity; ( C ) <t>Colorimetric</t> measurement of lactate concentration. ( D – G ) Real-time quantitative PCR analysis of mRNA levels for GLUT1, PKM2, LDHA, and MCT1; ( H – L ) Western blot analysis of protein expression levels for GLUT1, PKM2, LDHA, and MCT1. n=3; * P <0.05, ** P <0.01 compared to M0; # P <0.05, ## P <0.01 compared to M2.
Mouse Ifn Gamma Ifng Elisa Kit Boster Cat, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of CB on Glycolysis in M2 Macrophages. ( A ) Fluorescence images of macrophages after uptake of 2-NBDG (400×); ( B ) Quantification of average fluorescence intensity; ( C ) <t>Colorimetric</t> measurement of lactate concentration. ( D – G ) Real-time quantitative PCR analysis of mRNA levels for GLUT1, PKM2, LDHA, and MCT1; ( H – L ) Western blot analysis of protein expression levels for GLUT1, PKM2, LDHA, and MCT1. n=3; * P <0.05, ** P <0.01 compared to M0; # P <0.05, ## P <0.01 compared to M2.
Mouse Il6 Elisa Kit Boster Cat, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of CB on Glycolysis in M2 Macrophages. ( A ) Fluorescence images of macrophages after uptake of 2-NBDG (400×); ( B ) Quantification of average fluorescence intensity; ( C ) <t>Colorimetric</t> measurement of lactate concentration. ( D – G ) Real-time quantitative PCR analysis of mRNA levels for GLUT1, PKM2, LDHA, and MCT1; ( H – L ) Western blot analysis of protein expression levels for GLUT1, PKM2, LDHA, and MCT1. n=3; * P <0.05, ** P <0.01 compared to M0; # P <0.05, ## P <0.01 compared to M2.
E El M0676 Mouse Il 1 Beta Il1b Elisa Kit Boster Cat, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of CB on Glycolysis in M2 Macrophages. ( A ) Fluorescence images of macrophages after uptake of 2-NBDG (400×); ( B ) Quantification of average fluorescence intensity; ( C ) <t>Colorimetric</t> measurement of lactate concentration. ( D – G ) Real-time quantitative PCR analysis of mRNA levels for GLUT1, PKM2, LDHA, and MCT1; ( H – L ) Western blot analysis of protein expression levels for GLUT1, PKM2, LDHA, and MCT1. n=3; * P <0.05, ** P <0.01 compared to M0; # P <0.05, ## P <0.01 compared to M2.
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Proteintech sa00001
Effect of CB on Glycolysis in M2 Macrophages. ( A ) Fluorescence images of macrophages after uptake of 2-NBDG (400×); ( B ) Quantification of average fluorescence intensity; ( C ) <t>Colorimetric</t> measurement of lactate concentration. ( D – G ) Real-time quantitative PCR analysis of mRNA levels for GLUT1, PKM2, LDHA, and MCT1; ( H – L ) Western blot analysis of protein expression levels for GLUT1, PKM2, LDHA, and MCT1. n=3; * P <0.05, ** P <0.01 compared to M0; # P <0.05, ## P <0.01 compared to M2.
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Promega dual-luciferase reporter assay system
Effect of CB on Glycolysis in M2 Macrophages. ( A ) Fluorescence images of macrophages after uptake of 2-NBDG (400×); ( B ) Quantification of average fluorescence intensity; ( C ) <t>Colorimetric</t> measurement of lactate concentration. ( D – G ) Real-time quantitative PCR analysis of mRNA levels for GLUT1, PKM2, LDHA, and MCT1; ( H – L ) Western blot analysis of protein expression levels for GLUT1, PKM2, LDHA, and MCT1. n=3; * P <0.05, ** P <0.01 compared to M0; # P <0.05, ## P <0.01 compared to M2.
Dual Luciferase Reporter Assay System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Selleck Chemicals hy 12275 hdac inhibitor trichostatin a tsa selleck
Effect of CB on Glycolysis in M2 Macrophages. ( A ) Fluorescence images of macrophages after uptake of 2-NBDG (400×); ( B ) Quantification of average fluorescence intensity; ( C ) <t>Colorimetric</t> measurement of lactate concentration. ( D – G ) Real-time quantitative PCR analysis of mRNA levels for GLUT1, PKM2, LDHA, and MCT1; ( H – L ) Western blot analysis of protein expression levels for GLUT1, PKM2, LDHA, and MCT1. n=3; * P <0.05, ** P <0.01 compared to M0; # P <0.05, ## P <0.01 compared to M2.
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Effect of CB on Glycolysis in M2 Macrophages. ( A ) Fluorescence images of macrophages after uptake of 2-NBDG (400×); ( B ) Quantification of average fluorescence intensity; ( C ) <t>Colorimetric</t> measurement of lactate concentration. ( D – G ) Real-time quantitative PCR analysis of mRNA levels for GLUT1, PKM2, LDHA, and MCT1; ( H – L ) Western blot analysis of protein expression levels for GLUT1, PKM2, LDHA, and MCT1. n=3; * P <0.05, ** P <0.01 compared to M0; # P <0.05, ## P <0.01 compared to M2.
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Image Search Results


Effect of CB on Glycolysis in M2 Macrophages. ( A ) Fluorescence images of macrophages after uptake of 2-NBDG (400×); ( B ) Quantification of average fluorescence intensity; ( C ) Colorimetric measurement of lactate concentration. ( D – G ) Real-time quantitative PCR analysis of mRNA levels for GLUT1, PKM2, LDHA, and MCT1; ( H – L ) Western blot analysis of protein expression levels for GLUT1, PKM2, LDHA, and MCT1. n=3; * P <0.05, ** P <0.01 compared to M0; # P <0.05, ## P <0.01 compared to M2.

Journal: Drug Design, Development and Therapy

Article Title: Cinobufagin Inhibits Invasion and Migration of Non-Small Cell Lung Cancer via Regulating Glucose Metabolism Reprogramming in Tumor-Associated Macrophages

doi: 10.2147/DDDT.S531190

Figure Lengend Snippet: Effect of CB on Glycolysis in M2 Macrophages. ( A ) Fluorescence images of macrophages after uptake of 2-NBDG (400×); ( B ) Quantification of average fluorescence intensity; ( C ) Colorimetric measurement of lactate concentration. ( D – G ) Real-time quantitative PCR analysis of mRNA levels for GLUT1, PKM2, LDHA, and MCT1; ( H – L ) Western blot analysis of protein expression levels for GLUT1, PKM2, LDHA, and MCT1. n=3; * P <0.05, ** P <0.01 compared to M0; # P <0.05, ## P <0.01 compared to M2.

Article Snippet: A lactic Acid (LA) Colorimetric Assay Kit (Cat#:E-BC-K044-M; Elabscience) was used to measure lactic acid concentration in the cell culture supernatant.

Techniques: Fluorescence, Concentration Assay, Real-time Polymerase Chain Reaction, Western Blot, Expressing

Effect of CB on the Transcription Factor HIF-1α in M2 Macrophages. ( A and B ) Immunofluorescence analysis of HIF-1α nuclear translocation (400×); ( C and D ) Fluorescence images of macrophages after uptake of 2-NBDG (400×); ( E ) Colorimetric measurement of lactate concentration; ( F – J ) Western blot analysis of protein expression levels for GLUT1, PKM2, LDHA, and MCT1. n=3; * P <0.05 compared to M0; # P <0.05 compared to M2; $ P <0.05 compared to M2+DMOG.

Journal: Drug Design, Development and Therapy

Article Title: Cinobufagin Inhibits Invasion and Migration of Non-Small Cell Lung Cancer via Regulating Glucose Metabolism Reprogramming in Tumor-Associated Macrophages

doi: 10.2147/DDDT.S531190

Figure Lengend Snippet: Effect of CB on the Transcription Factor HIF-1α in M2 Macrophages. ( A and B ) Immunofluorescence analysis of HIF-1α nuclear translocation (400×); ( C and D ) Fluorescence images of macrophages after uptake of 2-NBDG (400×); ( E ) Colorimetric measurement of lactate concentration; ( F – J ) Western blot analysis of protein expression levels for GLUT1, PKM2, LDHA, and MCT1. n=3; * P <0.05 compared to M0; # P <0.05 compared to M2; $ P <0.05 compared to M2+DMOG.

Article Snippet: A lactic Acid (LA) Colorimetric Assay Kit (Cat#:E-BC-K044-M; Elabscience) was used to measure lactic acid concentration in the cell culture supernatant.

Techniques: Immunofluorescence, Translocation Assay, Fluorescence, Concentration Assay, Western Blot, Expressing