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Image Search Results
Journal: Drug Design, Development and Therapy
Article Title: Cinobufagin Inhibits Invasion and Migration of Non-Small Cell Lung Cancer via Regulating Glucose Metabolism Reprogramming in Tumor-Associated Macrophages
doi: 10.2147/DDDT.S531190
Figure Lengend Snippet: Effect of CB on Glycolysis in M2 Macrophages. ( A ) Fluorescence images of macrophages after uptake of 2-NBDG (400×); ( B ) Quantification of average fluorescence intensity; ( C ) Colorimetric measurement of lactate concentration. ( D – G ) Real-time quantitative PCR analysis of mRNA levels for GLUT1, PKM2, LDHA, and MCT1; ( H – L ) Western blot analysis of protein expression levels for GLUT1, PKM2, LDHA, and MCT1. n=3; * P <0.05, ** P <0.01 compared to M0; # P <0.05, ## P <0.01 compared to M2.
Article Snippet: A
Techniques: Fluorescence, Concentration Assay, Real-time Polymerase Chain Reaction, Western Blot, Expressing
Journal: Drug Design, Development and Therapy
Article Title: Cinobufagin Inhibits Invasion and Migration of Non-Small Cell Lung Cancer via Regulating Glucose Metabolism Reprogramming in Tumor-Associated Macrophages
doi: 10.2147/DDDT.S531190
Figure Lengend Snippet: Effect of CB on the Transcription Factor HIF-1α in M2 Macrophages. ( A and B ) Immunofluorescence analysis of HIF-1α nuclear translocation (400×); ( C and D ) Fluorescence images of macrophages after uptake of 2-NBDG (400×); ( E ) Colorimetric measurement of lactate concentration; ( F – J ) Western blot analysis of protein expression levels for GLUT1, PKM2, LDHA, and MCT1. n=3; * P <0.05 compared to M0; # P <0.05 compared to M2; $ P <0.05 compared to M2+DMOG.
Article Snippet: A
Techniques: Immunofluorescence, Translocation Assay, Fluorescence, Concentration Assay, Western Blot, Expressing